MAB5676

antibody from Novus Biologicals

Targeting: ITGA1 CD49a, VLA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5676

Provider

Novus Biologicals

Product name

Mouse Monoclonal Integrin alpha 1/CD49a Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/beta 4, alpha D/beta 2, alpha 7x2/beta 1, alpha E/beta 7, or alpha 2b/beta 3 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

α11β1 Integrin is Induced in a Subset of Cancer-Associated Fibroblasts in Desmoplastic Tumor Stroma and Mediates In Vitro Cell Migration.

Zeltz C, Alam J, Liu H, Erusappan PM, Hoschuetzky H, Molven A, Parajuli H, Cukierman E, Costea DE, Lu N, Gullberg D
Cancers 2019 Jun 1;11(6)

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Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3.

Chen WS, Cao Z, Sugaya S, Lopez MJ, Sendra VG, Laver N, Leffler H, Nilsson UJ, Fu J, Song J, Xia L, Hamrah P, Panjwani N
Nature communications 2016 Apr 12;7:11302

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Integrin alpha 1/CD49a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin alpha 1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.