Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-79525 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49a (Integrin alpha 1) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Sexual dimorphic impacts of systemic vincristine on lower urinary tract function.
Iguchi N, Hecht SL, Gao D, Wilcox DT, Malykhina AP, Cost NG
Scientific reports 2022 Mar 24;12(1):5113
Scientific reports 2022 Mar 24;12(1):5113
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD49a (Integrin alpha 1) in Lane 1: rat brain tissue lysate, Lane 2: HeLa whole cell lysate, Lane 3: SW620 whole cell lysate using 40-50 µg per well. Sample was incubated with CD49a (Integrin alpha 1) (Product # PA5-79525) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD49a (Integrin alpha 1) Polyclonal Antibody, (Product # PA5-79525) and a 200 kDa band corresponding to CD49a along with uncharacterized band at ~50 kDa was observed in the tissues tested with kidney showing higher expression compared to mammary gland. Whole cell extracts (40 µg lysate) of Mouse kidney (Lane 1), Mouse mammary gland (Lane 2), and Rat kidney (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD49a (Integrin alpha 1) on paraffin-embedded human intestinal cancer tissue. Sample was incubated with CD49a (Integrin alpha 1) polyclonal antibody (Product# PA5-79525).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD49a (Integrin alpha 1) on paraffin-embedded rat intestine tissue. Sample was incubated with CD49a (Integrin alpha 1) polyclonal antibody (Product# PA5-79525).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD49a (Integrin alpha 1) on paraffin-embedded human lung cancer tissue. Sample was incubated with CD49a (Integrin alpha 1) polyclonal antibody (Product# PA5-79525).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Gene expression analyses. The level of mRNA expression of each gene in the bladder ( A ) and Ls-DRG ( B ) is expressed as the fold difference to that in the control group. ( A ) normalized with a mean value of Gapdh and Tbp [N = 4-5 per group per sex] ( B ) normalized with Pgk1 . White and black bars represent the control and VCR groups, respectively. The upper and lower panels show the data for female and male mice, respectively. Mean +- SE. Representative Western blotting results ( C ) and the summary of changes normalized with Gapdh ( D ). Open and black bars represent the control and VCR groups, respectively [N = 3 per group per sex] Mean +- SE, fold difference (the control group taken as 1), *p < 0.05, **p < 0.005 vs. the control group. Figures were prepared using Adobe Photoshop CS6 and GraphPad Prism 8.4.3 ( https://www.graphpad.com/scientific-software/prism/ ).