MA5-42831

antibody from Invitrogen Antibodies

Targeting: RAP1GAP KIAA0474, RAP1GA1, RAP1GAP1, RAP1GAPII

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42831

Provider

Invitrogen Antibodies

Product name

RAP1GAP Recombinant Rabbit Monoclonal Antibody (8V9I6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: BxPC-3, U-87MG, Mouse brain, Rat brain. The target is usually found in the following locations: Golgi apparatus membrane, Peripheral membrane protein. Immunogen sequence: MIEKMQGSRM DEQRCSFPPP LKTEEDYIPY PSVHEVLGRE GPFPLILLPQ FGGYWIEGTN HEITSIPETE PLQSPTTKVK LECNPTARIY RKHFLGKEHF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

8V9I6

Vial size

100 µL

Concentration

0.66 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAP1GAP in extracts of various cell lines. Samples were incubated with RAP1GAP Monoclonal antibody (Product # MA5-42831) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RAP1GAP in NIH-3T3 cells using a RAP1GAP Recombinant Monoclonal antibody (Product # MA5-42831). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RAP1GAP in NIH-3T3 cells. Samples were incubated with RAP1GAP Monoclonal antibody (Product # MA5-42831) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.