PA5-82125

antibody from Invitrogen Antibodies

Targeting: SOX6

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-82125 - Provider product page
Provider: Invitrogen Antibodies
Product name: SOX6 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: QATSPFACAA DGEDAMTQDL TSREKEEGSD QHVASHLPLH PIMHNKPHSE ELPTLVSTIQ QDADWDSVLS SQQRMESENN KLCSLYSFRN TSTSPHKPDE GSRDREIMTS VTFGTPERRK GSLADVVDTL KQKKLEEMTR TEQ
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.2 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SOX6 protein structure - PA5-82125 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 6 FIGURE SOX6 is a downstream effector of miR-1290. (a) qRT-PCR showing the miR-1290 downregulation efficacy of miR-1290 inhibitor transfection. (b) Representative immunoblotting analysis revealing the transfection efficiency of si-SOX6 in suppressing SOX6. (c-j) ECA109 and KYSE450 cells were transiently introduced with si-SOX6 + miR-1290 inhibitor, si-con+miR-1290 inhibitor, miR-1290 inhibitor or inhibitor NC. (c) Representative immunoblotting analysis showing SOX6 protein level in transfected ECA109 and KYSE450 cells. (d) Proliferation analysis by CCK-8 assay with transfected ECA109 and KYSE450 cells. (e) Proliferation analysis by EDU assay with transfected ECA109 and KYSE450 cells. (f) Apoptosis assessment by flow cytometry with transfected ECA109 and KYSE450 cells. (g) Representative immunoblotting analysis showing Bax and Bcl-2 levels in transfected ECA109 and KYSE450 cells. (h and i) Representative migration (h) and invasion (i) analyses by transwell assay with transfected ECA109 and KYSE450 cells. (j) Migration evaluation by wound-healing assay with transfected ECA109 and KYSE450 cells. * p < 0.05

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 7 FIGURE Circ_0001946 affects tumor growth in vivo. (a-e) ECA109 cells were infected by circ_0001946-expressing lentivirus (lenti-circ_0001946) or control lentivirus (vector). 5 x 10 6 infected cells were hypodermically implanted into the left flanks of the BALB/c nude mice ( n = 5 per group). (a) Growth curves of the xenografts. Tumor growth was monitored for 4 weeks. (b) Images and average weight of the xenografts. (c) qRT-PCR showing the expression of circ_0001946, miR-1290 and SOX6 mRNA in the xenografts. (d) Representative immunoblotting analysis presenting SOX6 protein level in the xenografts. (e) Representative immunohistochemistry images showing ki-67, Bax, Bcl-2, MMP2, and MMP9 staining of sections of the xenografts. * p < 0.05