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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-86149 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86149, RRID:AB_11017119
- Product name
- Rabbit Polyclonal SOX6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SOX6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Yeung F, Chung E, Guess MG, Bell ML, Leinwand LA
Nucleic acids research 2012 Aug;40(15):7303-18
Nucleic acids research 2012 Aug;40(15):7303-18
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX6 Antibody [NBP1-86149] - Analysis in control (vector only transfected HEK293T lysate) and SOX6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX6 Antibody [NBP1-86149] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX6 Antibody [NBP1-86149] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX6 Antibody [NBP1-86149] - Staining in human testis and endometrium tissues using anti-SOX6 antibody. Corresponding SOX6 RNA-seq data are presented for the same tissues.
