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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-144306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZIC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis with a ZIC5 polyclonal antibody (Product # PA5-144306) using a dilution of 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
