HPA001924

antibody from Atlas Antibodies

Targeting: PPFIBP1 hSGT2, hSgt2p, L2, SGT2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001924

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001924, RRID:AB_1079665

Product name

Anti-PPFIBP1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DAQGFSDLEKSPSPTPVMGSPSCDPFNTSVPEEFH
TTILQVSIPSLLPATVSMETSEKSKLTPKPETSFE
ENDGNIILGATVDTQLCDKLLTSSLQKSSSLGNLK
KETSDGEKETIQKTSEDRA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines Caco-2 and HEK293 using Anti-PPFIBP1 antibody. Corresponding PPFIBP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line U-87 MG.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells and adnexal cells.

Sample type

HUMAN