PA1-28171

antibody from Invitrogen Antibodies

Targeting: AQP7 AQP7L, AQP9, AQPap

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA1-28171

Provider

Invitrogen Antibodies

Product name

Aquaporin 7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-28171 detects Aquaporin Adipose Specific from human samples.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Aquaporin 7 Polyclonal Antibody (Product # PA1-28171) and a 80 kDa band corresponding to Aquaporin 7 was observed in 3T3-L1 differentiated to adipocytes and Mouse Brown Adipose tissue and not in 3T3-L1, Mouse Adipose tissue, Mouse Brain and Mouse Skeletal Muscle which are reported negative for Aquaporin 7 expression. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to Adipocytes (Lane 2), tissue extracts (30 µg lysate) of Mouse Brown Adipose tissue (Lane 3), Mouse Adipose tissue (Lane 4), Mouse Brain (Lane 5) and Mouse Skeletal Muscle (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).