14-1038-80

antibody from Invitrogen Antibodies
Targeting: ITGAE CD103, HUMINAE
Provider product page for 14-1038-80
Immunohistochemistry
Flow cytometry

Antibody data

Product number
14-1038-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: The B-Ly7 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
B-Ly7
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
ITGAE protein structure - 14-1038-80 shown in red.