Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-1038-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: This B-Ly7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B-Ly7 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B-Ly7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits.
RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.
Schrøder M, Melum GR, Landsverk OJ, Bujko A, Yaqub S, Gran E, Aamodt H, Bækkevold ES, Jahnsen FL, Richter L
PloS one 2016;11(6):e0157387
PloS one 2016;11(6):e0157387
RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.
Jozwik A, Habibi MS, Paras A, Zhu J, Guvenel A, Dhariwal J, Almond M, Wong EHC, Sykes A, Maybeno M, Del Rosario J, Trujillo-Torralbo MB, Mallia P, Sidney J, Peters B, Kon OM, Sette A, Johnston SL, Openshaw PJ, Chiu C
Nature communications 2015 Dec 21;6:10224
Nature communications 2015 Dec 21;6:10224
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of unstimulated (blue histogram) or 3-day PHA-stimulated (purple histogram) normal human peripheral blood cells with Anti-Human CD103 (Integrin alpha E) APC. Total viable cells were used for analysis.