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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-34961 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PREX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: THP-1, human PREX1-transfected 293T cells. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PREX1 using 30 µg THP-1 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a PREX1 polyclonal antibody (Product # PA5-34961) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PREX1 Polyclonal Antibody (Product # PA5-34961). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PREX1 Polyclonal Antibody (Product # PA5-34961) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PREX1 Polyclonal Antibody detects PREX1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PREX1-transfected 293T cells.5 % SDS-PAGE. PREX1 Polyclonal Antibody (Product # PA5-34961) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PREX1 Polyclonal Antibody detects PREX1 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PREX1 Polyclonal Antibody (Product # PA5-34961) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
