Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB7087 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Sulfatase-2/SULF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Sulfatase-2/SULF2 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_940998
- Isotype
- IgG
- Antibody clone number
- 2B4
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment.
Zizza P, Dinami R, Porru M, Cingolani C, Salvati E, Rizzo A, D'Angelo C, Petti E, Amoreo CA, Mottolese M, Sperduti I, Chambery A, Russo R, Ostano P, Chiorino G, Blandino G, Sacconi A, Cherfils-Vicini J, Leonetti C, Gilson E, Biroccio A
Nucleic acids research 2019 Apr 23;47(7):3365-3382
Nucleic acids research 2019 Apr 23;47(7):3365-3382
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Sulfatase-2/SULF2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Sulfatase-2/SULF2 Monoclonal Antibody (Catalog # MAB7087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Sulfatase-2/SULF2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.