- 44-1140G - Invitrogen Antibodies FGFR1 antibody

Submitted references Frequent FGFR1 hotspot alterations in driver-unknown low-grade glioma and mixed neuronal-glial tumors.

Engelhardt S, Behling F, Beschorner R, Eckert F, Kohlhof P, Tatagiba M, Tabatabai G, Schuhmann MU, Ebinger M, Schittenhelm J
Journal of cancer research and clinical oncology 2022 Apr;148(4):857-866

A computationally identified compound antagonizes excess FGF-23 signaling in renal tubules and a mouse model of hypophosphatemia.

Xiao Z, Riccardi D, Velazquez HA, Chin AL, Yates CR, Carrick JD, Smith JC, Baudry J, Quarles LD
Science signaling 2016 Nov 22;9(455):ra113

Bone proteins PHEX and DMP1 regulate fibroblastic growth factor Fgf23 expression in osteocytes through a common pathway involving FGF receptor (FGFR) signaling.

Martin A, Liu S, David V, Li H, Karydis A, Feng JQ, Quarles LD
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2551-62

FRS2 via fibroblast growth factor receptor 1 is required for platelet-derived growth factor receptor beta-mediated regulation of vascular smooth muscle marker gene expression.

Chen PY, Simons M, Friesel R
The Journal of biological chemistry 2009 Jun 5;284(23):15980-92

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Lysates prepared from TT cells (lanes 1-5) were resolved by SDS-PAGE on a6% polyacrylamide gel and transferred to PVDF. Membranes were left untreated (lanes 1-4) or treated with lambda phosphatase (lane 5), blocked with a 3% BSA.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of FGFR1 (pYpY653/654) showing staining in the cytoplasm and membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a FGFR1 (pYpY653/654) polyclonal antibody (Product # 44-1140G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of FGFR1 (pYpY653/654) showing staining in the cytoplasm and membrane of paraffin-embedded human lung adenocarcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a FGFR1 (pYpY653/654) polyclonal antibody (Product # 44-1140G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

44-1140G

Antibody data