F50611

antibody from NSJ Bioreagents

Targeting: FGFR1 BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F50611

Provider

NSJ Bioreagents

Product name

FGFR1 Antibody

Antibody type

Polyclonal

Description

This highly specific FGFR1 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Flow cytometry/Western blot/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the FGFR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

The FGFR1 antibody used in western blot to detect FGFR1 in NIH3T3 cell lysate. Predicted molecular weight: 75-160 kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF: Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 7E3 and FGFR1 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background fluorescence.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human breast carcinoma tissue stained with the FGFR1 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 cells using FGFR1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.