H00002260-M03

antibody from Abnova Corporation

Targeting: FGFR1 BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002260-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002260-M03, RRID:AB_534865

Product name

FGFR1 monoclonal antibody (M03), clone 5E9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant FGFR1.

Antigen sequence

AQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSIN
WLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYA
CVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSS
SEEKETDNTKPNRMP


Isotype

IgG

Antibody clone number

5E9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FGFR1 expression in transfected 293T cell line by FGFR1 monoclonal antibody (M03), clone 5E9.Lane 1: FGFR1 transfected lysate(92 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FGFR1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FGF1 and FGFR1. HeLa cells were stained with anti-FGF1 rabbit purified polyclonal 1:1200 and anti-FGFR1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)