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LearnPAB0471
antibody from Abnova Corporation
Targeting: FGFR1
BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB0471 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0471, RRID:AB_983031
- Product name
- FGFR1 (phospho Y653/654) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y653/654 of human FGFR1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Glycated fibroblast growth factor-2 is quickly produced in vitro upon low-millimolar glucose treatment and detected in vivo in diabetic mice.
Facchiano F, D'Arcangelo D, Russo K, Fogliano V, Mennella C, Ragone R, Zambruno G, Carbone V, Ribatti D, Peschle C, Capogrossi MC, Facchiano A
Molecular endocrinology (Baltimore, Md.) 2006 Nov;20(11):2806-18
Molecular endocrinology (Baltimore, Md.) 2006 Nov;20(11):2806-18
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471) is used in Western blot to detect FGFR in mouse liver tissue lysate. FGFR (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR1 (arrow) using FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471). 293 cell lysates (2 &mciro;g/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
