H00002260-D01P

antibody from Abnova Corporation

Targeting: FGFR1 BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002260-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002260-D01P, RRID:AB_1674095

Product name

FGFR1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human FGFR1 protein.

Antigen sequence

MWSWKCLLFWAVLVTATLCTARPSPTLPEQDALPS
SEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKM
EKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKE
FKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCI
VENEYGSINHTYQLDVVERSPHRPILQAGLPANKT
VALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIG
PDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDA
GEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTS
PLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKS
DFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGV
LLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRD
RLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKV
AVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIIN
LLGACTQDGPLYVIVEYASKGNLREYLQARRPPGL
EYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS
KKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHI
DYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSF
GVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDK
PSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDR
IVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSG
EDSVFSHEPLPEEPCLPRHPAQLANGGLKRR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FGFR1 expression in transfected 293T cell line (H00002260-T03) by FGFR1 MaxPab polyclonal antibody.Lane 1: FGFR1 transfected lysate(81.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FGFR1 and CTNNB1. HeLa cells were stained with anti-FGFR1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)