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LearnNB100-2079
antibody from Novus Biologicals
Targeting: FGFR1
BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- NB100-2079 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2079, RRID:AB_10144921
- Product name
- Mouse Monoclonal FGFR1 Antibody
- Antibody type
- Monoclonal
Submitted references A homeo-interaction sequence in the ectodomain of the fibroblast growth factor receptor.
A homeo-interaction sequence in the ectodomain of the fibroblast growth factor receptor.
Wang F, Kan M, McKeehan K, Jang JH, Feng S, McKeehan WL
The Journal of biological chemistry 1997 Sep 19;272(38):23887-95
The Journal of biological chemistry 1997 Sep 19;272(38):23887-95
A homeo-interaction sequence in the ectodomain of the fibroblast growth factor receptor.
Wang F, Kan M, McKeehan K, Jang JH, Feng S, McKeehan WL
The Journal of biological chemistry 1997 Sep 19;272(38):23887-95
The Journal of biological chemistry 1997 Sep 19;272(38):23887-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.
