PA5-23353

antibody from Invitrogen Antibodies

Targeting: FGFR1 BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23353

Provider

Invitrogen Antibodies

Product name

Phospho-FGFR1 (Tyr651, Tyr652) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

20 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FGFR phospho in recombinant fusion protein containing 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652, using a Phospho-FGFR1 pTyr651+Y652 polyclonal antibody (Product # PA5-23353) at 0.002 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-FGFR1 (Tyr651, Tyr652) in FGFR phosphoin recombinant fusion protein. Samples were incubated in Phospho-FGFR1 (Tyr651, Tyr652) polyclonal antibody (Product # PA5-23353) using a dilution of 0.002 µg/mL followed by a goat anti-rabbit IgG HRP secondary antibody. 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652. PicoTect ECL substrate solution was used for this test.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-FGFR1 (Tyr651, Tyr652) in formalin-fixed, paraffin-embedded human breast tumor tissue. Samples were incubated in Phospho-FGFR1 (Tyr651, Tyr652) polyclonal antibody (Product # PA5-23353) using a dilution of 5 µg/mL. Isotype control antibody (top left) and FGFR1 phospho antibody (bottom left, right).