PA5-19787

antibody from Invitrogen Antibodies

Targeting: FST FS

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19787

Provider

Invitrogen Antibodies

Product name

Follistatin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

For Western Blot, this antibody has non-specific bands at 15 kDa and 75 kDa. This antibody is predicted to react with chicken, cow, dog, pig, chimpanzee and rhesus monkey based on sequence homology.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Skeletal Muscle Anti-Atrophic Effects of Leucine Involve Myostatin Inhibition.

Cruz A, Ferian A, Alves PKN, Silva WJ, Bento MR, Gasch A, Labeit S, Moriscot AS
DNA and cell biology 2020 Nov 2;

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Secretome profiling of PC3/nKR cells, a novel highly migrating prostate cancer subline derived from PC3 cells.

Jeon JM, Kwon OK, Na AY, Sung EJ, Cho IJ, Kim M, Yea SS, Chun SY, Lee JH, Ha YS, Kwon TG, Lee S
PloS one 2019;14(8):e0220807

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A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood.

Rebo J, Mehdipour M, Gathwala R, Causey K, Liu Y, Conboy MJ, Conboy IM
Nature communications 2016 Nov 22;7:13363

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Raji Whole Cell Lysate using Product # PA5-19787, Follistatin primary antibody at a dilution of 1 µg/mL (lane 1). Staining of MOLT4 Whole Cell Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 Validation of quantitative proteomics result using immunoblots. (A) Western blotting analysis of proteins with increased (Crk, SerpinB3, Cystatin-M), decreased (ULBP2), and unchanged secretion (Follistatin) in conditioned media (CM) and cell extract (CE) samples from PC3/nKR cells as compared with those from PC3 cells. (B) Densitometry results of Crk, SerpinB3, Cystatin-M, ULBP2 and Follistatin.