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LearnH00051343-M02
antibody from Abnova Corporation
Targeting: FZR1
CDC20C, CDH1, FZR, FZR2, HCDH, HCDH1, KIAA1242
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Proximity ligation assay [1]
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Validation data
Reference
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- Product number
- H00051343-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051343-M02, RRID:AB_1136937
- Product name
- FZR1 monoclonal antibody (M02), clone 4C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant FZR1.
- Antigen sequence
MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPAS
SPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKS
PSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIE
KVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPD
DGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIP
FKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVY
LWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVA
VGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWN
AEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQE
VCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQ
YTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFW
NTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGY
SQYQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDG
EAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLVT
RIR- Isotype
- IgG
- Antibody clone number
- 4C4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FZR1 monoclonal antibody (M02), clone 4C4. Western Blot analysis of FZR1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FZR1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to FZR1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CCNB1 and FZR1. HeLa cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-FZR1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
