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Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Other assay [1]
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- Product number
- 370506 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#370506, RRID:AB_2629600
- Product name
- Alexa Fluor® 594 anti-human Granzyme K
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- GM26E7
- Vial size
- 100 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood using a Ficol Gradient method. The PBMCs were washed and fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 0.5% FBS for 30 minutes. After blocking, the cells were incubated with Granzyme K (clone GM26E7) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for 30 minutes at room temperature. The cells were spun onto a microscopy slide with Cytospin. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
- Conjugate
- Yellow dye
