Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-8897-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granzyme K Monoclonal Antibody (G3H69), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This G3H69 monoclonal antibody reacts with human Granzyme K. Granzyme K is one of five granzyme serine proteases that have been indentified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Granzyme A and Granzyme K are both tryptases and appear to show overlapping, though not identical, substrate specificity. This functional similarity is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs.
- Antibody clone number
- G3H69
- Concentration
- 5 µL/Test
Submitted references Granzyme K displays highly restricted substrate specificity that only partially overlaps with granzyme A.
Residual cytotoxicity and granzyme K expression in granzyme A-deficient cytotoxic lymphocytes.
Bovenschen N, Quadir R, van den Berg AL, Brenkman AB, Vandenberghe I, Devreese B, Joore J, Kummer JA
The Journal of biological chemistry 2009 Feb 6;284(6):3504-12
The Journal of biological chemistry 2009 Feb 6;284(6):3504-12
Residual cytotoxicity and granzyme K expression in granzyme A-deficient cytotoxic lymphocytes.
Shresta S, Goda P, Wesselschmidt R, Ley TJ
The Journal of biological chemistry 1997 Aug 8;272(32):20236-44
The Journal of biological chemistry 1997 Aug 8;272(32):20236-44
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Normal human peripheral blood monocytes were stimulated for 3 days with Human IL-2 Recombinant Protein (Product # 14-8029-81), then cultured with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) for an additional 5 hours. The cells were stained with Anti-Human CD8a PE (Product # 12-0087-42) and Mouse IgG2a Isotype Control eFluor® 660 (Product # 50-4724-80) (left) or Anti-Human Granzyme K eFluor® 660 (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.