Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- ABIN108720 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108720, RRID:AB_10955340
- Product name
- anti-Granzyme K (Granzyme 3; Tryptase II) (GZMK) antibody
- Antibody type
- Monoclonal
- Antigen
- Our products GM24C3, GM26E7 and GM6C3 were generated by genetic immunisation of BALB/c mice with an immunisation vector containing the granzyme K cDNA. GM24C3, GM26E7 and GM6C3 recognise granzyme K transiently expressed on the cell surface of transfected
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GM24C3
- Vial size
- 100 ug
- Storage
- Store the antibody at 4°C. Avoid repeated freezing and thawing.
Submitted references Interleukin-4 suppresses the cytotoxic potential of in vitro generated, adaptive regulatory CD4 T cells by down-regulation of granzyme B.
Effect of pethidine premedication and halothane anaesthesia on upper limb blood flow.
Bratke K, Goettsching H, Kuepper M, Geyer S, Luttmann W, Virchow JC
Immunology 2009 Jul;127(3):338-44
Immunology 2009 Jul;127(3):338-44
Effect of pethidine premedication and halothane anaesthesia on upper limb blood flow.
Pauca AL
British journal of anaesthesia 1992 Jun;68(6):621-2
British journal of anaesthesia 1992 Jun;68(6):621-2
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using C3. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of C3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Intracellular detection of granzyme B in human PBMC. FACS analysis of human PBMC using hybridoma supernatant of C3. PBMC were cultivated in the presence of phorbolester and ionomycin subsequently fixed and permeabilised. Binding of C3 was detected with a FITC-conjugated secondary antibody.