AP6406a

antibody from Abgent

Targeting: PINK1 BRPK, PARK6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6406a

Provider

Abgent

Proper citation

Abgent Cat#AP6406a, RRID:AB_2164235

Product name

PINK1 (PARK6) Antibody (N-term T133)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of Park6(PINK1) (arrow) using rabbit polyclonal Park6(PINK1) Antibody (N-term) (Cat. #AP6406a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Park6(PINK1) gene.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Park6 (PINK1) Antibody (N-term) (Cat. #AP6406a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Park6 (PINK1) antibody detected the Park6 (PINK1) protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-PARK6 Pab (Cat. #AP6406a) in mouse kidney tissue lysate. PARK6 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100