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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- F40407 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PINK1 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific PINK1 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Western blot/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 38CT18.2.6
- Vial size
- 0.05 ml, 0.2 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the PINK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) A431 cell line and (2) mouse brain lysate using Pink1 antibody at 1:1000. Predicted molecular weight: 60-70 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PINK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene. Predicted molecular weight: 60-70 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of human 1) A431, 2) HeLa, 3) MCF7, 4) 293T and 5) mouse brain lysate with PINK1 antibody. Predicted molecular weight: 60-70 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of PC12 cells stained with Pink1 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488 secondary was used (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human heart using Pink1 antibody at 1:25 dilution.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach using Pink1 antibody at 1:25 dilution.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- PINK1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
