600-401-GU5

antibody from Invitrogen Antibodies

Targeting: PINK1 BRPK, PARK6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

600-401-GU5

Provider

Invitrogen Antibodies

Product name

PINK1 truncated Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit anti-PINK1 truncated antibody. Lane 1: recombinant PINK1. Load: 1 µg per lane. Primary antibody: PINK1 truncated antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody p/n (611-103-122) at 1:70,000 for 45 min at RT. Block: Odyssey blocking buffer overnight at 4°C. Predicted/Observed size: ~62, 30/~30 kDa for PINK1. Other band(s): PINK1 phosphorylates and experiences rapid degradation causing various bands on WB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit anti-PINK1 truncated antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: HEK293T untransfected lysate (W09-001-GX5). Lane 3: PINK1-overexpressing 293T. Load: 10 µg per lane. Primary antibody: PINK1 truncated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti-RABBIT IgG (H&L) (DONKEY) Antibody DyLight™ 488 Conjugated p/n (611-741-127) at 1:70,000 for 45 min at RT. Block: Odyssey blocking buffer overnight at 4°C. Predicted/Observed size: ~62, 30/~30 kDa for PINK1.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence Microscopy of Rabbit anti-PINK1 truncated antibody. Cell line: NIH 3T3 (p/n W10-000-358). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PINK1 truncated antibody at 20 µg/mL for overnight at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 µg/mL for 2 hrs at RT. Localization: PINK1 is mitochondrial and cytoplasmic. Staining: PINK1 as green fluorescent signal with DAPI (blue) nuclear counterstain.