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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA1-16625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PINK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has only been tested on transfected lysates. Endogenous protein detection is unknown.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of C-terminally V5His-tagged human Pink1 or N-terminally myc-tagged human Pink1 expressed in HEK293T cells. Product # PA1-16625 detects transfected Pink1 protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PINK1 in HEK293T cells. Samples were incubated in PINK1 polyclonal antibody (Product # PA1-16625 using a dilution of 1:1000. Observed molecular weight ~64 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PINK1 in 1.0 mg/mL HeLa lysate. Samples were incubated in PINK1 polyclonal antibody (Product # PA1-16625) using a dilution of 0.0763888888888889. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
