Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96539 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granzyme A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: Mouse spleen, Mouse thymus, Rat intestine, Rat spleen; Cellular Location: Cytoplasmic granule Immunogen sequence: IIGGNEVTPH SRPYMVLLSL DRKTICAGAL IAKDWVLTAA HCNLNKRSQV ILGAHSITRE EPTKQIMLVK KEFPYPCYDP ATREGDLKLL QLMEKAKINK YVTILHLPKK GDDVKPGTMC QVAGWGRTHN SASWSDTLRE VNITIIDRKV CNDRNHYNFN PVIGMNMVCA GSLRGGRDSC NGDSGSPLLC EGVFRGVTSF GLENKCGDPR GPGVYILLSK KHLNWIIMTI KGAV
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Granzyme A in extracts of various cell lines using Granzyme A Polyclonal Antibody (Product # PA5-96539) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Granzyme A in extracts of various cell lines using Granzyme A Polyclonal Antibody (Product # PA5-96539) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Granzyme A was performed in U2OS cells using Granzyme A Polyclonal Antibody (Product # PA5-96539) at a dilution of 1:100. Blue: DAPI for nuclear staining.
