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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- 507208 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#507208, RRID:AB_492996
- Product name
- Pacific Blue™ anti-human Granzyme A [CB9]
- Antibody type
- Monoclonal
- Antigen
- Purified human Granzyme A
- Description
- Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infection.
Proliferation capacity and cytotoxic activity are mediated by functionally and phenotypically distinct virus-specific CD8 T cells defined by interleukin-7R{alpha} (CD127) and perforin expression.
Yamamoto T, Johnson MJ, Price DA, Wolinsky DI, Almeida JR, Petrovas C, Nason M, Yeh WW, Shen L, Roederer M, Rao SS, McDermott AB, Lefebvre F, Nabel GJ, Haddad EK, Letvin NL, Douek DC, Koup RA
Journal of virology 2012 May;86(10):5877-84
Journal of virology 2012 May;86(10):5877-84
Proliferation capacity and cytotoxic activity are mediated by functionally and phenotypically distinct virus-specific CD8 T cells defined by interleukin-7R{alpha} (CD127) and perforin expression.
Cellerai C, Perreau M, Rozot V, Enders FB, Pantaleo G, Harari A
Journal of virology 2010 Apr;84(8):3868-78
Journal of virology 2010 Apr;84(8):3868-78
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blueâ¢
- Conjugate
- Blue dye
