TA505270

antibody from OriGene

Targeting: B3GNT2 B3GN-T1, B3GN-T2, B3GNT-2, B3GNT1, BETA3GNT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505270

Provider

OriGene

Proper citation

OriGene Cat#TA505270, RRID:AB_2622970

Product name

B3GNT2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Antibody type

Monoclonal

Description

B3GNT2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Host

Mouse

Conjugate

Unconjugated

Epitope

B3GNT2

Isotype

IgG

Antibody clone number

OTI1G10

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-B3GNT2 mouse monoclonal antibody (TA505270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505270).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC208100 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-B3GNT2 antibody(TA505270), and then analyzed by flow cytometry.

Validation comment

FC