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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- TA505283 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505283, RRID:AB_2622974
- Product name
- B3GNT2 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)
- Antibody type
- Monoclonal
- Description
- B3GNT2 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- B3GNT2
- Isotype
- IgG
- Antibody clone number
- OTI3A9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis.
Mkhikian H, Mortales CL, Zhou RW, Khachikyan K, Wu G, Haslam SM, Kavarian P, Dell A, Demetriou M
eLife 2016 Jun 8;5
eLife 2016 Jun 8;5
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-B3GNT2 mouse monoclonal antibody (TA505283) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505283).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
- Validation comment
- IHC
