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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-52023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHST7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PMWHLWQALY PGDAESLQGA LRDMLRSLFR CDFSVLRLYA PPGDPAARAP DTANLTTAAL FRWRTNKVIC SPPLCPGAPR ARAEVGLVED TACERSCPPV AIRALEAECR KYPVVVIKDV RLLDLGVLVP LLRDPGLNLK VVQL
- Concentration
- 0.05 mg/mL
Submitted references Osthole interacts with an ER-mitochondria axis and facilitates tumor suppression in ovarian cancer.
Bae H, Lee JY, Song J, Song G, Lim W
Journal of cellular physiology 2021 Feb;236(2):1025-1042
Journal of cellular physiology 2021 Feb;236(2):1025-1042
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CHST7 in human duodenum tissue shows moderate cytoplasmic and membranous positivity in glandular cells. Samples were probed using a CHST7 Polyclonal Antibody (Product # PA5-52023).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 8 Figure Osthole influenced intracellular mechanisms related to endoplasmic reticulum (ER)-mitochondrial functions. Expression of ER-mitochondrial function targets such as (a) ULK1, (b) GRP75, (c) MFN2, (d) P62, (e) FAM82A2, (f) VAPB, (g) VDAC, (h) IP3R1, and (i) IP3R2 was analyzed by western blot analysis in ES2 and OV90 cells treated with various doses of osthole (0, 5, 10, and 20 muM). Intensity of the target protein was detected and analyzed relative to total protein or alpha-tubulin protein. *** p < .001, ** p < .01, and * p < .05 indicate significant differences compared to vehicle-treated cells. IP3R1, inositol 1,4,5-trisphosphate (IP3) receptor type 1; IP3, inositol trisphosphate; MFN2, mitofusin-2; VAPB, vesicle-associated membrane protein-associated protein B/C; VDAC, voltage-dependent anion channel
