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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-148399 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin O Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.72 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of Cyclin O in Hela cells. Samples were incubated with Cyclin O polyclonal antibody (Product # PA5-148399) using a dilution of 1:200 at 4°C overnight followed by Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.
