14-6504-82

CSPG4 antibody from Invitrogen Antibodies
HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Immunocytochemistry
Supportive data in Antibodypedia
Immunoprecipitation
Recommended by provider
Immunohistochemistry
Recommended by provider
Flow cytometry
Recommended by provider

Antibody data

Product number
14-6504-82
Provider
Invitrogen Antibodies
Product name
Anti-Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), eBioscience™
Provider product page
Invitrogen Antibodies - 14-6504-82
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2, which is also known as melanoma chondroitin sulfate proteoglycan (MCSP). This antigen is composed of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan. NG2 is present on the surface of >90% of malignant melanomas in addition to some non-melanomic tumors. Moreover, NG2 is expressed on glioma cells, as well as on developing and adult oligodendrocyte precursor cells. Studies have demonstrated the involvement of this protein in tumor cell proliferation, adhesion, migration, and invasion. NG2 also functions as a coreceptor for alpha4beta1 integrin. Finally, expression of NG2 can be used as a prognostic marker for disorders such as acral letiginous melanoma and infantile acute myeloid leukemia. The 9.2.27 antibody has been reported to suppress melanoma tumor growth. Applications Reported: This 9.2.27 has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin embedded tissue (IHC-P), and immunocytochemical staining of fixed cells (ICC). Applications Tested: This 9.2.27 antibody has been tested by immunocytochemistry on methanol-fixed A375 cells at less than or equal to 10 ug/mL and by flow cytometric analysis on A375 cells at less than or equal to 0.125 ug/test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
9.2.27
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
Provider Type Product Number
- No reagents -