14829-1-AP

antibody from Invitrogen Antibodies

Targeting: MARS1 CMT2U, MARS, MetRS, SPG70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14829-1-AP

Provider

Invitrogen Antibodies

Product name

MARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LGNFINRAG MFVSKFFGGY VPEMVLTPDD QRLLAHVTLE LQHYHQLLEK VRIRDALRSI LTISRHGNQY IQVNEPWKRI KGSEADRQRA GTVTGLAVNI AALLSVMLQP YMPTVSATIQ AQLQLPPPAC SILLTNFLCT LPAGHQIGTV SPLFQKLEND QIESLRQRFG GGQAKTSPKP AVVETVTTAK PQQIQALMDE VTKQGNIVRE LKAQKADKNE VAAEVAKLLD LKKQLAVAEG KPPEAPKGKK KK (650-900 aa encoded by BC002384)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14829-1-AP (MARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14829-1-AP (MARS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14829-1-AP (MARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14829-1-AP (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 14829-1-AP (MARS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MARS (IP:14829-1-AP, 4ug; Detection:14829-1-AP 1:2000) with HepG2 cells lysate 4000ug.