Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-50056 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aldolase C Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. Also reacts with Aldolase A and B.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldolase C Antibody (E9) [NBP2-50056] - Right: Blots of rat brain lysate blotted with NBP2-50056. NBP2-50056 binds strongly and cleanly to a band at about 40 kDa. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots. Above: Blots of recombinant human Aldolase A, B and C with NBP2-50056 as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope for NBP2-50056 is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where Aldolase C specific antibodies bind. The specificity of two other Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide (clone 4A9) and one to the C-terminal peptide (clone 1A1).
