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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [5]
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Validation data
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- Product number
- LS-C798651 - Provider product page
- Provider
- LSBio
- Product name
- GATA3 Antibody (aa155-443, clone OTI8E11, Carrier-free) LS-C798651
- Antibody type
- Monoclonal
- Description
- Affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI8E11
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY GATA3. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3. (1:2000)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts. (35ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells using anti-GATA3 mouse monoclonal antibody. (Left). The right is negative control. (1:1000)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody. (Left). The right is negative control. (1:1000)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody. (Left). The right is negative control. (1:1000)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells using anti-GATA3 mouse monoclonal antibody. (Left). The right is negative control. (1:1000)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells using anti-GATA3 mouse monoclonal antibody. (Left). The right is negative control. (1:1000)
