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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C776075 - Provider product page
- Provider
- LSBio
- Product name
- HMOX2 / Heme Oxygenase 2 Antibody (Atto 700) LS-C776075
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Near infrared dye
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Mouse, Rat Brain showing detection of ~36 kDa HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: SPR-319. Lane 3: Mouse Brain. Lane 4: Rat Brain. Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~36 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody. Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody. Tissue: Prostate. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
