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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 14817-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMOX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSAEVETSE GVDESEKKNS GALEKENQMR MADLSELLKE GTKEAHDRAE NTQFVKDFLK GNIKKELFKL ATTALYFTYS ALEEEMERNK DHPAFAPLYF PMELHRKEAL TKDMEYFFGE NWEEQVQCPK AAQKYVERIH YIGQNEPELL VAHAYTRYMG DLSGGQVLKK VAQRALKLPS TGEGTQFYLF ENVDNAQQFK QLYRARMNAL DLNMKTKERI VEEANKAFEY NMQIFNELDQ AGSTLARETL EDGFPVHDGK GDMRKCPFYA AEQDKGALEG SSCPFRTAMA VLRK (1-293 aa encoded by BC002396)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP ( HMOX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using HMOX2 antibody 14817-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14817-1-AP (HMOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using HMOX2 antibody 14817-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14817-1-AP ( HMOX2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14817-1-AP ( HMOX2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HMOX2 (IP:14817-1-AP, 4ug; Detection:14817-1-AP 1:500) with Raji cells lysate 2000ug.
