Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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Validation data
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- Product number
- ADI-SPA-897-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-897-D, RRID:AB_2039196
- Product name
- HO-2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Protein A affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster, Porcine, Simian
- Host
- Rabbit
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Endogenous heme oxygenase prevents impairment of cerebral vascular functions caused by seizures.
Effects of hypoxia on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells.
Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene.
Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
Amyloid precursor proteins inhibit heme oxygenase activity and augment neurotoxicity in Alzheimer's disease.
Carratu P, Pourcyrous M, Fedinec A, Leffler CW, Parfenova H
American journal of physiology. Heart and circulatory physiology 2003 Sep;285(3):H1148-57
American journal of physiology. Heart and circulatory physiology 2003 Sep;285(3):H1148-57
Effects of hypoxia on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells.
Appleton SD, Marks GS, Nakatsu K, Brien JF, Smith GN, Graham CH, Lash GE
American journal of physiology. Heart and circulatory physiology 2003 Mar;284(3):H853-8
American journal of physiology. Heart and circulatory physiology 2003 Mar;284(3):H853-8
Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene.
Melo LG, Agrawal R, Zhang L, Rezvani M, Mangi AA, Ehsan A, Griese DP, Dell'Acqua G, Mann MJ, Oyama J, Yet SF, Layne MD, Perrella MA, Dzau VJ
Circulation 2002 Feb 5;105(5):602-7
Circulation 2002 Feb 5;105(5):602-7
Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
Sato K, Balla J, Otterbein L, Smith RN, Brouard S, Lin Y, Csizmadia E, Sevigny J, Robson SC, Vercellotti G, Choi AM, Bach FH, Soares MP
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 15;166(6):4185-94
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 15;166(6):4185-94
Amyloid precursor proteins inhibit heme oxygenase activity and augment neurotoxicity in Alzheimer's disease.
Takahashi M, Doré S, Ferris CD, Tomita T, Sawa A, Wolosker H, Borchelt DR, Iwatsubo T, Kim SH, Thinakaran G, Sisodia SS, Snyder SH
Neuron 2000 Nov;28(2):461-73
Neuron 2000 Nov;28(2):461-73
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of HO-2, pAb: Lane 1: MW marker, Lane 2: HO-2 Recombinant Human Protein (Prod. No. ADI-SPP-550), Lane 3: Human Liver Microsome Extract, Lane 4: Mouse Liver Microsome Extract, Lane 5: Rat Liver Microsome Extract, Lane 6: Dog Liver Microsome Extract.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human breast cancer tissue with HO-2 pAb.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunofluorescent analysis (confocal) staining of MCF-7 cells using HO-2 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.