Explore
Validate
Learn
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BML-HC3002-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-HC3002-0025, RRID:AB_2051580
- Product name
- Heme oxygenase 2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 25 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: HO-1 was detected using HO-1, pAb (Prod. no. BML-HC3001) (lanes 1, 3, 5 and 7), and HO-2 was detected using HO-2, pAb (Prod. no. BML-HC3002) (lanes 2, 4, 6 and 8). Both antibodies were used at a dilution of 1:1000. Luminograph (ECL, 15 sec. exposure, ref. FM020/054) showing HO-1 and HO-2 expression in lysates prepared from gIFN/LPS stimulated RAW264.7 mouse macrophage cells (lanes 1 and 2), HepG2 (lanes 3 and 4), rat testis (HP9312, lanes 5 and 6) and rat spleen (HP9313, lanes 7 and 8).
