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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB3170 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse HO-2/HMOX2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse HO-2/HMOX2 in Western blots. Endogenous rat HO-2 is detectable but has a higher background. In Western blots, no cross-reactivity with recombinant human HO-1/HMOX1 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P30519- Isotype
- IgG
- Antibody clone number
- 322913
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse HO-2/HMOX2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse HO-2/HMOX2 Monoclonal Antibody (Catalog # MAB3170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-2/HMOX2 was detected at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
