Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-39408 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RAB5C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB5C in extracts from HeLa cells, Jurkat cells and COLO cells using a RAB5C polyclonal antibody (Product # PA5-39408).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RAB5C was achieved by transfecting HEK 293 with RAB5C specific siRNAs (Silencer® select Product # s11708). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the RAB5C knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RAB5C Polyclonal Antibody (Product # PA5-39408, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAB5C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK293 (Lane 2), SK-BR-3 (Lane 3), A549 (Lane 4), A-431 (Lane 5), Caco-2 (Lane 6) and NIH3T3 (Lane 7). The blot was probed with Anti-RAB5C Polyclonal Antibody (Product # PA5-39408, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to RAB5C was observed across all the cell lines tested.