PA1-12521

antibody from Invitrogen Antibodies

Targeting: DAPK3 ZIP, ZIPK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12521

Provider

Invitrogen Antibodies

Product name

ZIPK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for this product is Jurkat or HeLa whole cell lysate.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ZIPK Polyclonal Antibody (Product # PA1-12521) and a 52 kDa band corresponding to DAPK3 was observed across cell lines tested except LNCaP which is reported to be low. A doublet banding pattern is observed, which is probably due to autophosphorylation of the DAPK3 which is reported in literatures. Modified whole cell extracts (1% SDS) (30 µg lysate) of DU 145 (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), U-87 MG (Lane 5) and Jurkat (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).