66017-1-IG

antibody from Invitrogen Antibodies

Targeting: TPP2 TPPII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

66017-1-IG

Provider

Invitrogen Antibodies

Product name

TPPII Monoclonal Antibody (5H6F11)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: LERLKDLPF IVSHRLSNTL SLDIHENHSF ALLGKKKSSN LTLPPKYNQP FFVTSLPDDK IPKGAGPGCY LAGSLTLSKT ELGKKADVIP VHYYLIPPPT KTKNGSKDKE KDSEKEKDLK EEFTEALRDL KIQWMTKLDS SDIYNELKET YPNYLPLYVA RLHQLDAEKE RMKRLNEIVD AANAVISHID QTALAVYIAM KTDPRPDAAT IKNDMDKQKS TLVDALCRKG CALADHLLHT QAQDGAISTD AEGKEEEGES PLDSLAETFW ETTKWTDLFD NKVLTFAYKH ALVNKMYGRG LKFATKLVEE KPTKENWKNC IQLMKLLGWT HCASFTENWL PIMYPPDYCV F (900-1249 aa encoded by BC039905)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5H6F11

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-IG (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of TPPII antibody (66017-1-IG, 1:4000) with si-control and si-TPPII transfected Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-mouse IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 66017-1-IG (TPPII antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 66017-1-IG (TPPII antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 66017-1-Ig (TPPII antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 66017-1-Ig (TPPII antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug TPPII antibody (66017-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.