Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12385-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12385-1-AP, RRID:AB_2211886
- Product name
- UBAP1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated UBAP1 antibody (Cat. #12385-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells.
Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.
A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions.
VPS37A directs ESCRT recruitment for phagophore closure.
The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.
A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.
ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.
Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.
The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR.
Serine Phosphorylation of HIV-1 Vpu and Its Binding to Tetherin Regulates Interaction with Clathrin Adaptors.
The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.
A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction.
The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.
UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.
microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network.
Lu D, Zhi Y, Su H, Lin X, Lin J, Shi Y, Yi W, Hong C, Zhang T, Fu Z, Chen LY, Zhao Z, Li R, Xu Z, Chen W, Wang N, Xu D
Cell reports 2024 Mar 26;43(3):113818
Cell reports 2024 Mar 26;43(3):113818
Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.
Kazan JM, Desrochers G, Martin CE, Jeong H, Kharitidi D, Apaja PM, Roldan A, St Denis N, Gingras AC, Lukacs GL, Pause A
iScience 2021 Nov 19;24(11):103274
iScience 2021 Nov 19;24(11):103274
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.
Parkinson G, Roboti P, Zhang L, Taylor S, Woodman P
Journal of cell science 2021 Nov 1;134(21)
Journal of cell science 2021 Nov 1;134(21)
A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions.
Yao H, Li C, He F, Song T, Brosseau JP, Wang H, Lu H, Fang C, Shi H, Lan J, Fang JY, Xu J
RSC chemical biology 2021 Feb 1;2(1):192-205
RSC chemical biology 2021 Feb 1;2(1):192-205
VPS37A directs ESCRT recruitment for phagophore closure.
Takahashi Y, Liang X, Hattori T, Tang Z, He H, Chen H, Liu X, Abraham T, Imamura-Kawasawa Y, Buchkovich NJ, Young MM, Wang HG
The Journal of cell biology 2019 Oct 7;218(10):3336-3354
The Journal of cell biology 2019 Oct 7;218(10):3336-3354
The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.
Miller DSJ, Bloxham RD, Jiang M, Gori I, Saunders RE, Das D, Chakravarty P, Howell M, Hill CS
Cell reports 2018 Nov 13;25(7):1841-1855.e5
Cell reports 2018 Nov 13;25(7):1841-1855.e5
A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.
Imbert PR, Louche A, Luizet JB, Grandjean T, Bigot S, Wood TE, Gagné S, Blanco A, Wunderley L, Terradot L, Woodman P, Garvis S, Filloux A, Guery B, Salcedo SP
The EMBO journal 2017 Jul 3;36(13):1869-1887
The EMBO journal 2017 Jul 3;36(13):1869-1887
ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.
Mamińska A, Bartosik A, Banach-Orłowska M, Pilecka I, Jastrzębski K, Zdżalik-Bielecka D, Castanon I, Poulain M, Neyen C, Wolińska-Nizioł L, Toruń A, Szymańska E, Kowalczyk A, Piwocka K, Simonsen A, Stenmark H, Fürthauer M, González-Gaitán M, Miaczynska M
Science signaling 2016 Jan 19;9(411):ra8
Science signaling 2016 Jan 19;9(411):ra8
Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.
Kharitidi D, Apaja PM, Manteghi S, Suzuki K, Malitskaya E, Roldan A, Gingras MC, Takagi J, Lukacs GL, Pause A
Cell reports 2015 Oct 20;13(3):599-609
Cell reports 2015 Oct 20;13(3):599-609
The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR.
Verma R, Marchese A
The Journal of biological chemistry 2015 Mar 13;290(11):6810-24
The Journal of biological chemistry 2015 Mar 13;290(11):6810-24
Serine Phosphorylation of HIV-1 Vpu and Its Binding to Tetherin Regulates Interaction with Clathrin Adaptors.
Kueck T, Foster TL, Weinelt J, Sumner JC, Pickering S, Neil SJ
PLoS pathogens 2015 Aug;11(8):e1005141
PLoS pathogens 2015 Aug;11(8):e1005141
The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.
Wunderley L, Brownhill K, Stefani F, Tabernero L, Woodman P
Journal of cell science 2014 Feb 1;127(Pt 3):663-72
Journal of cell science 2014 Feb 1;127(Pt 3):663-72
A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction.
Kueck T, Neil SJ
PLoS pathogens 2012;8(3):e1002609
PLoS pathogens 2012;8(3):e1002609
The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.
Agromayor M, Soler N, Caballe A, Kueck T, Freund SM, Allen MD, Bycroft M, Perisic O, Ye Y, McDonald B, Scheel H, Hofmann K, Neil SJ, Martin-Serrano J, Williams RL
Structure (London, England : 1993) 2012 Mar 7;20(3):414-28
Structure (London, England : 1993) 2012 Mar 7;20(3):414-28
UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.
Stefani F, Zhang L, Taylor S, Donovan J, Rollinson S, Doyotte A, Brownhill K, Bennion J, Pickering-Brown S, Woodman P
Current biology : CB 2011 Jul 26;21(14):1245-50
Current biology : CB 2011 Jul 26;21(14):1245-50
microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network.
Zhang L, Deng T, Li X, Liu H, Zhou H, Ma J, Wu M, Zhou M, Shen S, Li X, Niu Z, Zhang W, Shi L, Xiang B, Lu J, Wang L, Li D, Tang H, Li G
Carcinogenesis 2010 Apr;31(4):559-66
Carcinogenesis 2010 Apr;31(4):559-66
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP(UBAP1 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using UBAP1 antibody 12385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 12385-1-AP(UBAP1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
