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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- PA5-47444 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- A33 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of A33 in immersion fixed paraffin-embedded sections of human colon. Samples were incubated in A33 polyclonal antibody (Product # PA5-47444) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TH29 human colon carcinoma cell line was stained with human A33 Antigen Affinitypurified Polyclonal (Product # PA5-47444, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of A33 in TH-29 human colon carcinoma cell line. Samples were incubated in A33 polyclonal antibody (Product # PA5-47444) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of A33 in TH-29 human colon carcinoma cell line. Samples were incubated in A33 polyclonal antibody (Product # PA5-47444) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
