F55008

antibody from NSJ Bioreagents

Targeting: GPA33 A33

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55008

Provider

NSJ Bioreagents

Product name

GPA33 Antibody / Cell surface A33 antigen

Antibody type

Polyclonal

Description

This highly specific GPA33 antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the GPA33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) 293T and 2) HT-29 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562, 2) HEK293 and 3) MDA-MB-231 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma tissue with GPA33 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-231 cells with GPA33 antibody; Blue=isotype control, Green= GPA33 antibody.