AF1213

antibody from R&D Systems

Targeting: JAM3 JAM-C, JAMC

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF1213

Provider

R&D Systems

Product name

Mouse JAM-C Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM-C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9D8B7


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis.

Hintermann E, Bayer M, Ehser J, Aurrand-Lions M, Pfeilschifter JM, Imhof BA, Christen U
Cell adhesion & migration 2016 Jul 3;10(4):419-33

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Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus.

Wyss L, Schäfer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams RH, Aurrand-Lions M, Plate KH, Imhof BA, Engelhardt B
PloS one 2012;7(9):e45619

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Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.

Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
Investigative ophthalmology & visual science 2009 Mar;50(3):1454-63

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Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.

Fuse C, Ishida Y, Hikita T, Asai T, Oku N
The Journal of biological chemistry 2007 Mar 16;282(11):8276-83

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Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.

Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schön MP, Kaufmann R, Boehncke WH, Podda M
The Journal of investigative dermatology 2005 Nov;125(5):969-76

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

JAM-C in Mouse Splenocytes. JAM-C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

JAM-C in Mouse Embryo. JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.